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. Author manuscript; available in PMC: 2017 May 23.
Published in final edited form as: J Androl. 2011 Jul 14;33(3):309–337. doi: 10.2164/jandrol.111.014167

Figure 4.

Figure 4

Male germinal granules (intermitochondrial cement/nuage and chromatoid bodies) highlighted by immunofluorescent staining of mouse VASA homolog (MVH [DDX4]) and murine PIWI homolog 1 (MIWI [PIWIL1]) in adult mouse testes. Intermitochondrial cement (arrow) is widely distributed in the cytoplasm of all spermatogenic cells, including spermatogonia (Sg), spermatocytes (Sp), and spermatids (Sd), whereas chromatoid bodies (arrowheads) resemble perinuclear bodies mainly found in round spermatids (rSd). MVH (red fluorescence) is associated with male germinal granules in all types of developing germ cells, including intermitochondrial cement in all spermatogenic cells and chromatoid bodies in round spermatids. MIWI (green fluorescence) is predominantly expressed in intermitochondrial cement in pachytene spermatocytes and chromatoid bodies in round spermatids. eSd, elongated spermatids. Purified human anti-MVH/DDX4 antibody (R11-A3 Fab) was purchased from BD Pharmingen Inc (San Diego, California), and affinity-purified rabbit anti-PIWIL1 polyclonal antibodies were prepared by GenScript Corporation (Piscataway, New Jersey). Both antibodies were used at a dilution of 1:100.