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. 2005 Jan;4(1):72–81. doi: 10.1128/EC.4.1.72-81.2005

FIG. 2.

FIG. 2.

Activities of amylase and glycogen phosphorylase in cell lines with different extracellular CF activity. countin mutant, Ax4 (WT), and smlA mutant cells were starved by shaking in PBM and harvested at 6 h of development, and the activities of amylase and glycogen phosphorylase were measured. (A) Maltose was used to prepare a calibration curve for reducing sugar produced by amylase. Values are means ± standard error of the mean from six independent assays. The lines show Michaelis-Menten equations fit to the data with nonlinear regression. The Vmax of wild-type cells was 7.7 nmol/mg of protein/min, and the Km was 7.7 mg/ml. (B) The activity of glycogen phosphorylase was measured by coupling the reaction to NADH production. Values are means ± standard error of the mean from five independent assays. The lines show Michaelis-Menten equations fit to the data with nonlinear regression. The Vmax of wild-type cells was 1.7 nmol/mg of protein/min, and the Km was 1.3 mg/ml.