TABLE 1.

Levels of glycolytic intermediates^a

Enzyme	Mean concn (μmol/g of packed cells) ± SEM
	smlA mutant	Wild type	countin mutant
Glucose-6-phosphate	0.13 ± 0.02	0.080 ± 0.008	0.050 ± 0.006
Fructose-6-phosphate	0.052 ± 0.004	0.030 ± 0.003	0.023 ± 0.002
Fructose-1,6-bisphosphate	0.47 ± 0.04	0.63 ± 0.05	0.66 ± 0.07

^aCells were starved by shaking in PBM for 6 h, and the levels of glucose-6-phosphate, fructose-6-phosphate, and fructose-1,6-bisphosphate were measured. T-tests indicated that the levels of glucose-6-phosphate in mutant cells were significantly different from those in wild-type cells, with P < 0.02. In addition, the levels of fructose-6-phosphate and fructose-1,6-bisphosphate in smlA cells were significantly different from those in wild-type cells with P < 0.005 and P < 0.03, respectively. Values are means from eight (glucose-6-phosphate and fructose-6-phosphate) or nine (fructose-1,6-bisphosphate) independent assays.