TABLE 1.
Enzyme | Mean concn (μmol/g of packed cells) ± SEM
|
||
---|---|---|---|
smlA mutant | Wild type | countin mutant | |
Glucose-6-phosphate | 0.13 ± 0.02 | 0.080 ± 0.008 | 0.050 ± 0.006 |
Fructose-6-phosphate | 0.052 ± 0.004 | 0.030 ± 0.003 | 0.023 ± 0.002 |
Fructose-1,6-bisphosphate | 0.47 ± 0.04 | 0.63 ± 0.05 | 0.66 ± 0.07 |
Cells were starved by shaking in PBM for 6 h, and the levels of glucose-6-phosphate, fructose-6-phosphate, and fructose-1,6-bisphosphate were measured. T-tests indicated that the levels of glucose-6-phosphate in mutant cells were significantly different from those in wild-type cells, with P < 0.02. In addition, the levels of fructose-6-phosphate and fructose-1,6-bisphosphate in smlA cells were significantly different from those in wild-type cells with P < 0.005 and P < 0.03, respectively. Values are means from eight (glucose-6-phosphate and fructose-6-phosphate) or nine (fructose-1,6-bisphosphate) independent assays.