TABLE 3.

V_max and K_m of glucose-6-phosphate after exposure to countin^a

Cells	Vmax (nmol/mg of protein/mm)	Km (mM)
Countin treated	0.62	0.48
Control	0.44	0.073

^aThe V_max and K_m of glucose-6-phosphatase in washed wild type cells treated with recombinant countin were determined. Wild-type cells were starved by shaking in PBM and harvested at 6 h of starvation. Cells were washed in PBM, resuspended, and treated with bovine serum albumin (control) or recombinant countin for 1 min and collected, and the activity of glucose-6-phosphatase was measured (Fig. 6B). Because of the time required to collect cells by centrifugation, the total exposure time to recombinant countin was ∼8 mins. The V_max and K_m values were calculated as in Table 2.