TABLE 3.
Cells | Vmax (nmol/mg of protein/mm) | Km (mM) |
---|---|---|
Countin treated | 0.62 | 0.48 |
Control | 0.44 | 0.073 |
The Vmax and Km of glucose-6-phosphatase in washed wild type cells treated with recombinant countin were determined. Wild-type cells were starved by shaking in PBM and harvested at 6 h of starvation. Cells were washed in PBM, resuspended, and treated with bovine serum albumin (control) or recombinant countin for 1 min and collected, and the activity of glucose-6-phosphatase was measured (Fig. 6B). Because of the time required to collect cells by centrifugation, the total exposure time to recombinant countin was ∼8 mins. The Vmax and Km values were calculated as in Table 2.