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. Author manuscript; available in PMC: 2017 May 23.
Published in final edited form as: Nature. 2017 Feb 27;543(7644):211–216. doi: 10.1038/nature21358

Extended Data Figure 9. TIRR depletion increases radiosensitivity.

Extended Data Figure 9

a, Whole cell extracts from RPE-1 cells (CTRL) and from a polyclonal population (poly) and three RPE-1 clones deprived of endogenous TIRR using CRISPR/cas9 system were analyzed by immunoblotting using the indicated antibodies. The amount of 53BP1 was quantified and normalized to β-tubulin using ImageJ. b, Kinetics of γH2AX foci formation in indicated cells after a 2 Gy irradiation. The graph represents the mean number of γH2AX foci per cell (mean ± s.d., n=3). c, Clonogenic survival of indicated cells following irradiations (0 to 5 Gy). TIRR expression has been restored in clone#13 by stable expression of GFP-TIRR. Survival was expressed as a percentage of colonies formed relative to the non-irradiated control (mean ± s.d., n=3).