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. Author manuscript; available in PMC: 2017 May 23.
Published in final edited form as: Nature. 2017 Feb 27;543(7644):211–216. doi: 10.1038/nature21358

Extended Data Figure 2. Ectopic TIRR restores RAD51 foci formation in BRCA1-mutant MEFs.

Extended Data Figure 2

a, Brca1-mutant MEFs stably transduced with an empty vector (EV) or TIRR-FH were transfected with the indicated siRNA. RAD51 was stained six hours after 5 Gy irradiation (mean ± s.d., n=3). b, Quantification of CSR at 96h or 72h post-stimulation from splenic B cells transduced with pMIG or pMIG-TIRR, and stimulated with LPS and IL-4 for 72 h. c, AID, Sμ germline (Sμ GLT) and Sγ1 germline (Sγ1 GLT, black) transcripts in splenic B cells stimulated for 48 h (mean ± s.d., n=3 mice). d, Immunoblotting of AID and TIRR from lysates prepared from splenic B cells stimulated for 72 h.