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S-1-P increases resistance of the sgk-null mutants to cisplatin. S-1-P at the indicated concentrations was added to growing cultures of the parental Ax4 cells and the sgk-null mutant cells 1 h prior to the addition of 150 μM cisplatin. The cells were harvested after 5 h and plated for viability. Symbols: ○, S-1-P alone; •, S-1-P at the indicated concentrations plus 150 μM cisplatin.
