FIG. 6.
Sod2 is required for virulence. (A) Groups of 10 A/Jcr mice were anesthetized by intraperitoneal injection of pentobarbital and infected with 5 × 105 yeast cells of strains sod2Δ, sod2Δ SOD2, and wild-type (WT) H99 via intranasal inhalation. The sod2Δ mutant strain was avirulent: all infected animals survived to 80 days postinfection. Reconstitution of the sod2Δ mutant strain restored virulence. By day 38, all of the mice infected with strain sod2Δ SOD2 had succumbed to infection. All mice infected with wild-type strain H99 had succumbed to infection by day 25.