TABLE 1.
Primers used in this study
| Primer name | Usea | Sequence (5′-3′) |
|---|---|---|
| sgSOD2 OL-1 | A, D | GGTGGGTGATTACAGGTTGG |
| sgSOD2 OL-2 | A, D | GGTCGAGCAACTTCGCTCCATATGACCACCACCGTTGA |
| sgSOD2 OL-3 | A | CCACCTCCTGGAGGCAAGTTGTCTATCTTCGGGTGTGG |
| sgSOD2 OL-4 | A | GAAGGCGCACAAGGTAAGAG |
| sgSOD2 OL-5 | A | TCAACGGTGGTGGTCATATGGAGCGAAGTTGCTCGACC |
| sgSOD2 OL-6 | A | CCACACCCGAAGATAGACAACTTGCCTCCAGGAGGTGG |
| sgSOD2 P-5′ | B | CTCCGCACTTCTCTCGCTAC |
| sgSOD2 P-3′ | B | CGTTCAAATCCACACACCTG |
| sgSOD2(rc)-OL1 | C | GGTGGGTGATTACAGGTTGG |
| sgSOD2(rc)-OL2 | C | GCTCACCTCCCGCAGCCTGAAGGCGCACAAGGTAAGAG |
| sgSOD2(rc)-OL3 | C | CTCTTACCTTGTGCGCCTTCAGGCTGCGGGAGGTGAGC |
| sgSOD2(rc)-OL4 | C | GAAGAGATGTAGAAACGAGTTTC |
a
The primers were used for the following purposes: A, PCR-OL construction of the sod2Δ::URA5 construct; B, colony PCR detection of sod2Δ mutant strains; C, PCR-OL construction of the SOD2-nat construct; D, probe for Southern blot analysis.