Fig 1. Histatin-1 improves rates of wound closure in an in vitro scratch assay.

(A) Representative images from wound healing assay of HCLE cell cultures treated with histatin-1 demonstrating enhancement of wound closure compared to “vehicle only” (PBS) control; scale bar = 200μM (only 10 μM shown) (B) Summary bar graph illustrating percentage wound closure at indicated time points during the scratch assay. Notable is the statistically significant improvement in wound closure times. Each condition was compared to “vehicle only” (PBS) control. P values: ***P ≤ 0.001 as determined by two-way ANOVA with Bonferroni’s posttest. The error bar represents means ± SEM of three scratched areas imaged per condition.