MD map of the E. coli chromosome are represented with the position of the Ori-4, Right-2, FROS-Tag in an Inv (A), or oriCZ (B) configurations. For each panel, the position of foci in cell containing 2 foci (cell with one focus are show in S4 Fig), are represent in function of the long axis of the cell (y-axis) and in function of the cell length (x-axis). Red dots and bars correspond to the localization of the Right-2 loci and green ones to the Ori-4 loci. (C) Histogram of the percentage of the population presenting different interfocal distance (<0.2 to > 0.5) between Ori-4 and Right-2 in the WT strain (blue), Inv strain (red) and oriCZ strain (green). Numbers on the first columns show the percentage of cells where both foci are co-localized.