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. 2017 Apr 24;114(19):5047–5052. doi: 10.1073/pnas.1618557114

Fig. 5.

Fig. 5.

NIP1;2-facilitated root Al uptake requires a functional ALMT1-mediated root malate exudation system. (A) Al3+ ions, but not Al-Mal, are retained in the root cell wall. Here 7-d-old seedlings were treated with AlCl3 or Al-Mal (pH 4.2) for 8 h, after which Al concentrations in the root cell wall (A) and root cell sap (B) were measured by ICP-MS. (C and D) Externally supplied malate partially restored NIP1;2-mediated Al uptake in the almt1 KO mutant. Here 7-d-old seedlings were pretreated with AlCl3 (pH 4.2) for 8 h, washed three times with 0.5 mM CaCl2 , and then treated with 200 μM malate (−Al) for 8 h. Al concentrations in the root cell wall (C) and root cell sap (D) were determined by ICP-MS. Data are mean ± SD of three sample replicates from three magenta boxes. In A and B, *P < 0.05, **P < 0.01) between WT and almt1 or nip1;2–1 under the indicated treatment conditions; in C and D, *P < 0.05 between two treatments for almt1.