Fig. S3.

Myo7b variants deficient in USH1C binding exhibit decreased colocalization with CDHR2. Confocal images of 14 d post confluency Myo7b KD CACO-2BBE cells expressing the EGFP-tagged Myo7b rescue constructs (A: EGFP-Myo7b-FL; B: EGFP-Myo7b-ΔCMF; C: EGFP-Myo7b-K1918E; D: EGFP-Myo7b-R1921E) indicated (green) and stained for CDHR2 (magenta) and F-actin (gray). (Scale bars, 10 μm.) The colocalization analysis of Myo7b constructs with CDHR2 using Pearson’s correlation coefficients is shown in Fig. 3C.