Fig. 4.

Crkl exon 2 deletion results in dose-dependent intrauterine growth restriction and Crkl exon2Δ/exon2Δ embryos exhibit 23% incidence of upper-tract phenotypes. Crkl^+/+, Crkl+/exon2Δ, and Crkl exon2Δ/exon2Δ embryos were collected at E16.5 (A), E17.5, and E18.5. Loss of one copy of wild-type Crkl is sufficient to confer intrauterine growth restriction as early as E16.5 (B). n for each group ≥ 7. Error bars represent SEM. GU tracts were microdissected from E16–E18. Upper-tract phenotypes were observed and penetrance documented (Inset chart). Phenotypes included mild (C) to moderate (D and F) hydronephrosis, unilateral renal agenesis (E), and unilateral renal hypoplasia. Asterisks indicate abnormalities. Asterisk in table indicates P < 0.05 by Fisher exact test. (Scale bars, 2 mm.)