Fig. S5.

A subset of genes differentially expressed between wild-type and homozygous mutants by RNA-sequencing were assayed by qPCR to compare expression between wild-type and heterozygous mutants. RNAs from the same samples used for sequencing were reverse-transcribed and used in TaqMan qPCR to interrogate differential expression. Although all of the genes shown here were differentially expressed between wild-type and homozygous mutants, no differences were apparent when comparing wild-type and heterozygous mutants. Biological triplicates; error bars represent SEM.