Fig. S2.

Effects of several factors on H⁺/ATP-ratio and $\mathrm{\Delta }{\mathit{G}}_{\mathit{p}}^{0^{\mathit{\text{'}}}}$. (A) Chemical potential of ATP synthesis vs. electrochemical potential of H⁺ at the point of equilibrium (no net ATP synthesis/hydrolysis). All data of the present study are plotted. Values of Δψ were calculated by the Nernst equation (gray, the same as Fig. 3) using K⁺ concentrations, [K⁺]_in and [K⁺]_out, (magenta) using the activities of K⁺ instead of the concentrations, or (green) after taking into account the increase in [K⁺]_in caused by K⁺ influx to PLs upon addition of valinomycin. Plots were fitted with a linear function by the least squares method. (B) The traces in A are extrapolated to their y-intercept region. The resulting coefficient of determination (R²), n value (H⁺/ATP-ratio),y and $\mathrm{\Delta }{G}_p^{0^{\text{'}}}$ are shown beside of the traces.