Fig. S2.

Sequence alignment of TtuA and related proteins. The amino acid sequence of TthTtuA is aligned to the sequences of other TtuA/Ncs6 proteins from Aquifex aeolicus, Thermotoga maritima, P. horikoshii, Homo sapiens, Saccharomyces cerevisiae, and M. maripaludis. The amino acid sequence of E. coli TtcA is also aligned. The secondary structure of TthTtuA is shown at the top. The completely conserved residues are highlighted in red, the highly conserved residues are highlighted in yellow, the PP-loop is indicated by the green line, the three Fe-S cluster-binding Cys residues are indicated by red asterisks, and the other Cys residues involved in Zn binding are indicated by blue asterisks.