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(A) Difference Fourier map of the bound [4Fe-4S] cluster in the TtuA-4Fe4S structure. The map is contoured at 6.5 σ. (B) Difference Fourier map of the bound AMPPNP in the TtuA-FeS-AMPPNP structure. The map is contoured at 4.5 σ. (C) Close-up view of the catalytic site of the TthTtuA-TtuB (G65C) complex. The Fe-S cluster is absent, and the two terminal residues of TtuB (shown by a red dotted line) are disordered.
