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. 2017 Apr 24;114(19):4954–4959. doi: 10.1073/pnas.1615585114

Table S1.

In vitro mutational analysis of TtuA

Mutations	Amount of Fe,^* pmol Fe/pmol TtuA	Enzyme activity^*		s²T amounts in vivo,^† %^‡
Mutations	Amount of Fe,^* pmol Fe/pmol TtuA	pmol s²T/pmol TtuA/min	%^‡	s²T amounts in vivo,^† %^‡
WT	3.84 ± 0.08	0.912 ± 0.030	100.0	100.0 ± 2.35
C130S	3.27 ± 0.09	0.067 ± 0.009	7.3	9.63 ± 0.31
C133S	2.82 ± 0.08	0.004 ± 0.001	0.4	0.81 ± 0.12
C222S	3.07 ± 0.45	0.011 ± 0.001	1.2	4.53 ± 2.56
C130S, C133S	2.41 ± 0.36	Not detected	0	Not determined
C130S, C222S	1.60 ± 0.11	Not detected	0	Not determined
C133S, C222S	1.64 ± 0.12	Not detected	0	Not determined
C130S, C133S, C222S	1.41 ± 0.14	Not detected	0	Not determined

^*

Experiments were repeated in triplicate.

^{^†}

The result of in vivo mutational analysis of TtuA (18) are presented.

^{^‡}

The values of WT were set to 100.