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. 2017 Apr 24;114(19):4869–4874. doi: 10.1073/pnas.1609278114

Fig. 2.

Fig. 2.

Flexible control of light sheets. (A and B) Top and side views of one arm of the illumination system. The scanning mirror is used to direct the light sheet along two axes as indicated by the dashed lines. (C) Top view of three objective lenses, which lie in a single plane. The two illumination lenses are centered on the common focal point of three lenses and rotated by an angle of ∼25° relative to the focal plane of the detection lens. (D) Top view of the two light sheets. The two scanning mirrors are used complementarily to guide the two light sheets along different angles (52°, 25°, and 0°). (E) Side view of two light sheets recorded through the front window of the specimen chamber. The chamber is filled with 50 μM Rose Bengal in PBS buffer to visualize the fluorescence emission generated by the light sheets with a 543-nm HeNe laser (25-LGP-193–230, Melles Griot). Two light sheets are rotated by an angle of 45° relative to the normal axis of the focal plane. The top view of the directions of two light sheets in E is identical to the image in the middle of D.