Fig. 3.
Long-range S3 spectra of Pf1 enabled assignment of distant side-chain sites, whereas comparison with short-range S3 helped to identify nearest-neighbor backbone correlations, as shown in 13C–13C planes (A) and 13C–15N planes (B) at the indicated 15N and 13C indirect dimension frequency, respectively, from short-range (green) and long-range (orange) CX(Cα)N(C′)CX i→i − 1 S3 spectra. DARR transfers of 10 and 50 ms were used for the short-range experiment; 50- and 250-ms DARR were used for long-range S3. 13C and 15N linewidths were as low as 1.0 and 3.5 ppm, respectively. See SI Appendix for additional experimental details.