Fig. 1.

Dnmt3a^R878H/WT mice develop AML characterized by expansion of HSPCs. (A, Upper) Macroscopic appearance of spleen and lymphoid nodes obtained from mice. No lymphadenopathy was observed in WT animals. (Lower) Kaplan–Meier survival curves of Dnmt3a^WT/WT (n = 15) and Dnmt3a^R878H/WT (n = 10) mice. (B) Morphological analysis of BM and spleen (SP) obtained from mice. Wright’s staining was performed on BM and SP cytospin preparations. (C) Statistical analysis of the number of immature cells and lymphocytes in the BM. (D and E) Flow cytometric quantification of mature hematopoietic cell populations and the indicated HSPC populations in BM cells of mice. Mean ± SEM values are shown. *P < 0.05, **P < 0.01, ***P < 0.001.