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. Author manuscript; available in PMC: 2017 May 23.
Published in final edited form as: Curr Mol Pharmacol. 2015;8(1):22–31. doi: 10.2174/1874467208666150507103215

Fig. (4).

Fig. (4)

Exon substitution strategy employed to elucidate the functional and behavioral contributions of e37a and e37b of Cacna1b. Mutually exclusive e37a and e37b are the same length but about 30% of amino acids they encode differ. E37b was replaced by a second copy of e37a, in the e37a-only mouse strain, and e37a was replaced by a second copy of e37b, in the e37b-only mouse strain. E37a is repressed in most region of the nervous system but is found at higher levels in dorsal root ganglia, e37b is expressed throughout the nervous system. E37a does not substitute for e37b in the e37a-only mouse, there is increased e37 lacking CaV2.2 mRNA in neurons, and significantly reduced CaV2.2 protein levels. Whereas, in the e37b-only mouse, e37b is inserted in place of e37a and wild-type levels of CaV2.2 protein is preserved [108, 120].