Fig. 1.
(A) Three unique amino acid substitutions were identified in HvGBSSIaCDC_Alamo (D219V, M490V, and I491V) as compared with a number of other waxy and non-waxy (in bold) barley varieties. (B) Mapping of these three amino acid substitutions on the structure of the catalytic domain of rice GBSSIa (Momma and Fujimoto, 2012; PDB entry 4VUF). D219V is located on the N-terminal Rossman fold while M490V and I491V are on the C-terminal Rossman fold. None of the three amino acids are positioned in the catalytic site in which ADP is bound, or involved in any other putative substrate, or protein binding sites.