Fig. 2.

(A–F) Subcellular localization of HvGBSSIa^WT-eGFP (A–C) and HvGBSSIa^CDC_Alamo-eGFP (D–F) in 100 µm-thick sections of developing endosperm (30 DAP) of grains from transgenic barley plants. Starch granules were detected with transmitted light using differential interference contrast (DIC) settings (A, D); HvGBSSIa^CDC_Alamo-eGFP and HvGBSSIa^WT-eGFP were detected as green epifluorescence (B, E); cell walls were detected as blue epifluorescence (C, F). HvGBSSIa^WT-eGFP is strictly targeted to starch granules (B), whereas HvGBSSIa^CDC_Alamo-eGFP is mostly targeted into unknown subcellular bodies (E). (G) Western blotting with GBSSI antiserum confirms that HvGBSSIa is strictly targeted to starch granules in the endosperm of Golden Promise, whereas it is mostly found in the supernatant of the endosperm cell fraction of CDC Alamo. All lanes are from the same blot.