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. 2017 Feb 11;68(5):931–941. doi: 10.1093/jxb/erw503

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© The Author 2017. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 5. — (A–H) eGFP fluorescence from endosperm cells (A, C, E, G) and iodine-stained starch granules (B, D, F, H) from developing grains (30 DAP) of plants with transgenic expression of HvGBSSIa^WT-eGFP (A, B, E, F) or HvGBSSIa^CDC_Alamo-eGFP (C, D, G, H) in the three waxy cultivars CDC Alamo (A–D), CDC Candle (E–H) and SB94912. The results for SB94912 were identical to those of CDC Candle, so for simplicity, only CDC Candle is shown. The scale bars in (A–H) are 50 µm. (I) Relative gene expression levels of HvGBSSIa determined by qPCR in developing grains (10 and 25 DAP) from plants with overexpression of either HvGBSSIa^WT-eGFP or HvGBSSIa^CDC_Alamo-eGFP (two different lines: 12 and 5) in the cultivars CDC Alamo and CDC Candle. (J) Amylose content in starch isolated from dry mature grains of plants expressing either HvGBSSIa^WT-eGFP or HvGBSSIa^CDC_Alamo-eGFP in CDC Alamo or CDC Candle.