Fig. 2.

Genetic and physical maps of the SPL33 gene. (A) The SPL33 gene was located on chromosome 1 between InDel markers I1-1 and S1-1-5. (B) The SPL33 gene was delimited to the V54-V19 interval using 148 F₂ mutant individuals; marker names and number of recombinants are shown. (C) Fine genetic mapping of the SPL33 gene based on 476 mutant F₂ individuals. (D) Eleven putative ORFs were located in an ~70-kb region. (E) Gene structure LOC_Os01g02720. Eleven exons and ten introns are indicated by green rectangles and black lines, respectively; a G to T point mutation was identified in the seventh exon (red arrow) generating a premature termination codon. (F) Sequence analysis of the G-to-T mutation site in plants of wild type and spl33.