Table 2.

Enzymes involved in the putative reductive acetyl-CoA pathway in T. ammonificans HB-1, closest relative homolog and homologs within the Aquificae phylum.

DOI: http://dx.doi.org/10.7554/eLife.18990.011

					Desulfurobac teriaceae		Hydrogenothe rmaceae		Aquificaceae
Reaction	Putative enzyme	Putative gene locus	Closest relative	Putative origin‡	D. thermolitho trophum	Desulfuro bacterium sp. TC5-1	P. marina	Sulfurihydro genibium sp. YO3AOP1	H. themophilus	Hydrogeno baculum sp. Y04AAS1H	Hydrogeni virga sp. 128–5 R1-6	T. ruber	A. aeolicus
1	Formate dehydrogenase	Theam_1020	Nitratiruptor sp. SB155-2 sim. 55% YP_001357016	Methanogens	f.e.§ - 30% YP_00428203#	-**	56% YP_002730364	f.e.§ - 32% YP_001930236	f.e.§ - 33% YP_003433330	-	-	f.e.§ - 27% YP_003474076	-
2*	5-formyltetra hydrofolate cyclo-ligase	Theam_1206	Hydrogenivirga sp. 128–5 R1-1 sim. 41% WP_008285842	Deltaproteo bacteria / Gram +	41% YP_004281339	44% WP_022846876.1	f.e.§ - 36% YP_002729868	f.e.§ - 38% YP_001931834	f.e.§ - 38% YP_003431710	f.e.§ - 30% YP_002121539	41% WP_008285842	40% YP_003472981	43% 1SOU_A
	formyltetra hydrofolate deformylase / hydrolase	Theam_0826	Hydrogenivirga sp. 128–5 R1-1 sim. 70% WP_008287030	Bacteria	82% YP_004281077	77% WP_022846479	-	-	-	-	70% WP_008287030	65% NP_214247	-
	phosphori bosylglycina mide formyl transferase 2	Theam_1211	P. marina sim. 76% YP_002731257	Methanogens / Deltaproteo bacteria	78% YP_004281335	64% WP_022847512	76% YP_002731257	73% YP_001931730	f.e.§ - 28% YP_003433072	67% YP_007499479	74% WP_008288476	f.e.§ - 25% YP_003473050	f.e.§ - 30% NP_213168
	phosphori bosylaminoi midazole carboxamide formyltransferase /IMP cyclohydrolase	Theam_0328	P. marina sim. 59% YP_002731268	Aquificae /  Bacteria	86% YP_004281681	78% WP_022847359	59% YP_002731268	56% YP_001931239	53% YP_003433270	50% YP_007499473	57% WP_008288360	52% YP_003474337	56% NP_214344
3	methylenetetra hydrofolate  dehydrogenase / methenyltetra hydrofolate cyclohydrolase	Theam_1261	A. aeolicus sim. 60% NP_214304	Aquificae / Gram +	87% YP_004281147	74% WP_022846217	55% YP_002731476	58% YP_001931240	59% YP_005511318	58% YP_001931240	-	58% YP_003473104	60% NP_214304
4	methylenetetra hydrofolate reductase	Theam_0919	Methanosrcina barkeri sim. 48% YP_305816	Methanogens	87% YP_004281147	74% WP_022846217	55% YP_002731476	58% YP_001931240	59% YP_005511318	58% YP_002121558	f.e.§ - 24% WP_008286783	58% YP_003473104	60% NP_214304
5	5-methyltetra hydrofolate corrinoid/iron sulfurprotein  methyltransferase	Theam_1184	Clostridium glycolicum sim. 42% WP_018589788	Gram +	82% YP_004281484	72% WP_022846603	f.e.§ - 31% YP_002729885	f.e.§ - 38% YP_001930381	f.e.§ - 37% YP_003432344	f.e.§ - 34% YP_002122031	f.e.§ - 38% WP_008287326	f.e.§ - 36% YP_003473438	f.e.§ - 34% NP_213375
6†	Carbon-monoxide  dehydrogenase	Theam_1337	Candidatus Methano peredens sp. BLZ1 sim. 56% KPQ43483	Archaea / Gram +	87% WP_013638427 38% WP_013638030	76% WP_022847143	41% YP_002729904	-	-	-	-	-	-

* – Reactions are numbered according to Figure 1. For reaction two we reported the possible enzymes that could substitute the missing 10-fomyl-THF synthetase (Fhs). The enzymes are listed in order of decreasing likelihood of their involvement in the reaction based on putative substrate affinity.

† – Reaction six is catalyzed by the Acs/CodH complex, reported here separately.

‡ – Putative origin of the gene was calculated as consensus taxonomic assignment of the first 100 bastp hits against the nr database. Double assignment implies equal number of assignments to the two taxonomic groups.

§ – f.e. = functional equivalent annotated in the genome with similarity below 40% with T. ammonificans equivalent gene. Not considered a true homolog in the present study.

# – Pairwise similarity to T. ammonificans translated gene and accession number of the homologs.

** – Missing homolog or functional annotated equivalent in the genome.