Author response image 2. Growth rates of rich medium evolved strains under nutrient limitation.
(left) is data reproduced from Figure 3 of the main text. (center) growth rates for founder (1) strain and strains after 5, 10 and 15 rounds of selection in 10-fold diluted LB medium. (right) identical measurements in 100-fold diluted LB medium. Declines in growth rate due to selection in 10- and 100-fold dilutions are statistically significant by linear regression (coefficient (confidence interval)):−0.019 [1/h* round] (−0.022, −0.015); −0.019 [1/h*round] (−0.031, −0.0005).