Table 3.
Pigment profiling of knock-down strains grown in control light (CL) and high light (HL)
Pigment amounts quantified by HPLC are normalized to 100 chlorophyll.
| Nx | Vx | Ax | Lut | Zx | β-Car | Chl a/b | Car/Chl | DI | Chl/cell | |
|---|---|---|---|---|---|---|---|---|---|---|
| WT CL | 9.3 ± 1.2 | 4.2 ± 0.5 | 0.27 ± 0.03 | 7.5 ± 0.9 | 0.22 ± 0.03 | 4.6 ± 0.1 | 2,19 ± 0,03 | 0.26 ± 0.02 | 0.076 ± 0.002 | 2,7E-06 ± 6,7E-08 |
| L6_A CL | 10.2 ± 0.04 | 4.9 ± 0.01 | 0.3 ± 0.01 | 8.4 ± 0.03 | 0.34 ± 0.03 | 5.01 ± 0.08 | 2,23 ± 0,05 | 0.29 ± 0.002 | 0.089 ± 0.004 | 2,0E-06 ± 1,7E-07 |
| L6_B CL | 9.4 ± 1.2 | 4.7 ± 0.8 | 0.3 ± 0.06 | 8.4 ± 1.1 | 0.16 ± 0.06 | 5.2 ± 2.1 | 2,26 ± 0,02 | 0.28 ± 0.05 | 0.064 ± 0.016 | 2,7E-06 ± 2,8E-07 |
| L_468 CL | 8.7 ± 1.1 | 4.2 ± 0.5 | 0.27 ± 0.04 | 7.4 ± 0.9 | 0.25 ± 0.05 | 4.8 ± 0.1 | 2,36 ± 0,02 | 0.26 ± 0.03 | 0.08 ± 0.005 | 2,9E-06 ± 4,0E-07 |
| WT HL | 7.7 ± 2.3 | 4.4 ± 1.6 | 1.4 ± 0.5 | 16.1 ± 4.8 | 1.7 ± 0.8 | 2.7 ± 1.9 | 2,22 ± 0,00 | 0.35 ± 0.14 | 0.316 ± 0.01 | 1,5E-06 ± 7,7E-08 |
| L6_A HL | 7.1 ± 1.6 | 5.1 ± 1.4 | 0.9 ± 0.1 | 15.4 ± 2,0 | 0.9 ± 0.003 | 1.4 ± 0.001 | 2,31 ± 0,01 | 0.35 ± 0.0 | 0.196 ± 0.03 | 1,4E-06 ± 1,1E-07 |
| L6_ B HL | 6.6 ± 0.9 | 4.9 ± 0.8 | 0.74 ± 0.1 | 13.2 ± 1.8 | 0.74 ± 0.1 | 4.99 ± 0.2 | 2,31 ± 0,07 | 0.31 ± 0.04 | 0.172 ± 0.0003 | 1,7E-06 ± 1,7E-07 |
| L_468 HL | 8.1 ± 1.4 | 5.7 ± 0.8 | 0.7 ± 0.08 | 14.2 ± 2.1 | 0.6 ± 0.04 | 4.5 ± 1.5 | 2,39 ± 0,00 | 0.34 ± 0.06 | 0.135 ± 0.002 | 2,0E-06 ± 2,1E-07 |
Nx, neoxanthin; Vx, violaxanthin; Ax, antheraxanthin, Lut, lutein, Zx, zeaxanthin, β-Car, β-carotene, Chl a/b, the ratio between Chl a and Chl b; Car/Chl, the ratio between the total carotenoid and chlorophyll content; DI, de-epoxidation index: DI=Zx+(0.5×Ax)/(Vx+Ax+Zx).
The SD is reported in the table (n=3).