Fig. 2.

Western blot analysis of leaf protein extracts from wild-type (WT) and bifunctional FBP/SBPase-expressing (FS) plants. Proteins were extracted from 2014 leaf tissue samples (R5, beginning seed developmental stage) of plants grown under the ‘Ac’ (400 μmol mol⁻¹ [CO₂], control temperature), ‘Ah’ (400 μmol mol⁻¹ [CO₂], heated +3.5 °C), ‘Ec’ (600 μmol mol⁻¹ [CO₂], control temperature), and ‘Eh’ (600 μmol mol⁻¹ [CO₂], heated +3.5 °C) treatments. A total of three leaflets (from three different plants) were combined per sample. The blot was probed at the same time with polyclonal antibodies raised against transketolase (TK) and native SBPase, and was reprobed using a polyclonal antibody raised against bifunctional FBP/SBPase after the blot was stripped. Gels were loaded on an equal protein basis. Blot marker images were cropped and pasted onto the corresponding chemiluminescence images using the editing function of the FusionCapt Advance software.