Fig. 3.

Constitutive expression of Actinidia CEN- and BFT-like genes affects flowering in Arabidopsis. (A) The range of flowering time recorded in T₁ lines grown in inductive LD conditions. Five to eight lines per construct were monitored for flowering time, recorded as the number of rosette leaves when the primary inflorescence stems were 0.2 cm long. Each dot represents the flowering time of at least one primary transgenic line. (B) Transgene expression in representative late flowering lines, normalized to ACT2. (C) Representative late flowering plants, of the same age and grown under the same conditions as the control Col-0 plant. (D) Examples of altered inflorescence morphology: proliferation of coflorescences on a primary inflorescence stem of a 35S:AcCEN2 plant, leafy axillary shoot produced on a 35S:AcBFT1 plant with abnormal flowers on the primary inflorescence stem, and indeterminate inflorescence meristems of 35S:AcCEN4 and 35S:AcBFT2 plants that failed to produce flowers. Arrows indicate flowers with leaf-like sepals. (E) Examples of inflorescences with whorled phyllotaxis of leaf-like organs (arrowheads) and small abnormal flowers (arrows).