Effect of growth irradiance on photosynthesis during moderate drought in WT tobacco and transgenic lines with altered amounts of AOX protein. (A) Anet. (B) ETRnet. (C) EPnet. (D) NPQnet. Plants were grown at 150, 400, or 700 PPFD under well-watered conditions for 19 to 21 days, followed by water being withheld from the plants until they were experiencing a leaf RWC of 63–66%, at which time each of the photosynthetic parameters was measured at the growth irradiance (150, 400, or 700 PPFD). Data are shown for WT plants (gray bars), AOX overexpressors (left to right the two black bars are B8 and B7, respectively) and AOX knockdowns (left to right the two white bars are RI9 and RI29, respectively). Data are the average ±SE of three to five independent experiments. Within an irradiance, values significantly different from the WT are indicated: *P<0.05; **P<0.01; ***P<0.001.