Table 1.
SGAT overexpressors grow slower than the wild type in normal air but wild-type-like at 1% CO2
Plant were grown to stage 5.1 (Boyes et al., 2001). Values are mean±SD from at least six biological replicates per genotype. Numbers marked with an asterisk are statistically significant from the wild type based on Student’s t-test (*P < 0.05).
| Growth parameter | Col-0 | L1 | L2 | L3 |
|---|---|---|---|---|
| Normal air (0.039% CO2) | ||||
| Diameter (cm) | 15.4 ± 1.37 | 15.1 ± 1.76 | 16.2 ± 2.20 | 14.8 ± 2.23 |
| Leaf number | 62.3 ± 3.01 | 52.5 ± 2.74* | 53.0 ± 6.42* | 46.2 ± 4.49* |
| Rosette area (cm–2) | 115 ± 4.45 | 90.3 ± 4.99* | 90.2 ± 6.93* | 84.0 ± 1.53* |
| Fresh weight (g) | 5.79 ± 0.63 | 5.03 ± 0.35* | 5.00 ± 0.60* | 5.14 ± 0.22* |
| Dry weight (g) | 0.49 ± 0.05 | 0.42 ± 0.06 | 0.44 ± 0.04 | 0.48 ± 0.04 |
| FW/DW ratio | 12.1 ± 0.61 | 11.4 ± 0.95 | 11.2 ± 0.63 | 10.7 ± 1.12* |
| Elevated CO2 (1% CO2) | ||||
| Diameter (cm) | 14.9 ± 1.51 | 14.9 ± 1.36 | 14.5 ± 1.04 | 13.2 ± 2.14 |
| Leaf number | 32.8 ± 6.52 | 36.2 ± 4.36 | 36.5 ± 2.89 | 36.5 ± 6.12 |
| Rosette area (cm–2) | 206 ± 15.2 | 222 ± 10.9 | 215 ± 8.48 | 209 ± 4.37 |
| Fresh weight (g) | 2.83 ± 0.87 | 3.08 ± 0.61 | 2.88 ± 0.39 | 2.35 ± 0.87 |
| Dry weight (g) | 0.23 ± 0.06 | 0.27 ± 0.06 | 0.28 ± 0.04 | 0.30 ± 0.07 |
| FW/DW ratio | 11.7 ± 2.89 | 11.8 ± 3.69 | 10.5 ± 1.36 | 9.44 ± 0.48 |