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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Chromosome Res. 2016 Dec 19;25(2):101–113. doi: 10.1007/s10577-016-9541-9

Figure 3. CLAMP regulates transcript abundance of roX differentially in males and females.

Figure 3

(A) The average log2 fold change for roX1 and roX2 abundance as measured by qRT-PCR in homozygous clamp2 females indicates that females have a significant increase in the abundance of both roX1 and roX2, while homozygous clamp2 males have a significant decrease in roX2 abundance. Shown is the average log2 fold change (ΔΔCt) of three biological replicates for roX1 and roX2 after normalization to three internal genes and compared to the respective sex of w−; clamp2; P{CLAMP} transgenic animals as in Figure 2A. (Error bars are +/− 1 S.E.M., **p<0.01, *p<0.05).

(B) The samples from Figure 3A were normalized to w−; clamp2; P{CLAMP} rescue males and show that roX1 transcript abundance in homozygous clamp2 females is statistically indistinguishable from males. The abundance of roX2 in homozygous clamp2 females is statistically 16-fold depleted compared to w−; clamp2; P{CLAMP} rescue males. (Error bars are +/− 1 S.E.M., **p<0.001, *p<0.005).