TABLE 1.
Strains and plasmids
| Strain or plasmid | Relevant characteristics | Reference or source |
|---|---|---|
| C. glutamicum strains | ||
| ATCC 13032 | Wild type | American Type Cul- ture Collection |
| ΔilvN | ilvN deletion | This work |
| ΔilvA ΔpanB | ilvA and panB deletions | This work |
| ilvNM13 | Mutation M13 in ilvN | This work |
| ΔilvA ΔpanB ilvNM13 | ilvA and panB deletions, muta- tion in ilvN | This work |
| Plasmids | ||
| pK19 | E. coli vector, Kmr | 17 |
| pK19B | E. coli vector, Kmr, no BglII site | This work |
| pKK5 | ilvBNC in pJC1 | 8 |
| pEC71 | E. coli-C. glutamicum vector, Kmr | 15 |
| pECKA | E. coli-C. glutamicum vector, Kmr | This work |
| pECKAilvBNC | ilvBNC in pECKA | This work |
| pK18mobsacB | E. coli vector for integration, Kmr | 23 |