TABLE 2.
Oligonucleotide primers
| Primer | 5′→3′ sequencea | Purpose |
|---|---|---|
| MILVNF | GCGGAGGAAGTACTGCC | ilvN mutation |
| MILVNR | CAATCAGATTAATTGCTGTTTA | ilvN mutation |
| ILVNM1 | GGACGTAGACGGTGACATTTCCCGCG | ilvN mutation |
| AT | ||
| MISBGL | GTTTAGAACTTGGCCGGAG | ilvN mutation |
| ILVNM13F | AGGACGTGACGATGACTTTTCCCGCGTAT | ilvN mutation |
| ILVNM13R | ATACGCGGGAAAAGTCATCGTCTACGTCCT | ilvN mutation |
| DELN1F | CTGAATTCCGGTGGACTGGGCACC | ilvN deletion |
| DELN1R | TTGAATGCGCTCTCTCCTTATGCCTCGGTC | ilvN deletion |
| DELN2F | TAAGGAGAGAGCGCATTCAACCTCGTGTCC | ilvN deletion |
| DELN2R | CCGAATTCAAGGAGAGGTCAGCGTC | ilvN deletion |
| DELPANBF1 | AACTGCAGCGCAAGAAGGTCT | panB deletion |
| DELPANBR1 | AATCGTCTGCATCGCGACCCAGCACAACGT | panB deletion |
| DELPANBF2 | GGGTCGCGATGCAGACGATTCGACGCATTG | panB deletion |
| DELPANBR2 | AACTGCAGGGGATTGACAA | panB deletion |
| DELILVAF1 | AGGAATTCAGAGGACTGAGCCTG | ilvA deletion |
| DELILVAR1 | TGGTTCGATAGGCCACGCCCTGGGCATGGT | ilvA deletion |
| DELILVAF2 | GGGCGTGGCCTATCGAACCAGCGGGAGCAG | ilvA deletion |
| DELILVAR2 | AAGAATTCGTACTCAGGAGTGCC | ilvA deletion |
a
Mutated bases are in boldface; restriction sites (ScaI, EcoRI, or PstI) are in italics.