TABLE 3.
Inhibition of activity of C. glutamicum wild-type AHAS by branched-chain amino acids
| Presence of inhibitor (5 mM)
|
Residual activity (%)a
|
|||
|---|---|---|---|---|
| Valine | Isoleucine | Leucine | Measuredb | Calculatedc |
| + | − | − | 44 | |
| − | + | − | 61 | |
| − | − | + | 62 | |
| + | + | − | 43 | 26.8 |
| + | − | + | 42 | 27.3 |
| + | + | + | 43 | 16.7 |
a
The activity without addition of any inhibitor is considered 100%.
b
The values are averages of at least three independent measurements; the standard deviations were within ± 7%.
c
Calculated for cumulative inhibition. This activity was calculated as follows: activity (percent) = [1−(percent inhibition with valine/100)] × [1−(percent inhibition with leucine/100)] × [1−(percent inhibition with isoleucine/100)] × 100.