TABLE 2.
PCR amplification of the HAE1 and PCS gene fragments from reference strainsa
| Strain | HAE1 geneb | PCRc
|
Reference | |
|---|---|---|---|---|
| A24f/A577r | PCS1f/PCS2r | |||
| Synechocystis sp. strain PCC 6803 | − | − | − | 17 |
| Sinorhizobium meliloti | + | + | − | 5 |
| Bacillus subtilis | − | − | − | 12 |
| Geobacter sulfurreducens | + | + | − | 16 |
| Streptomyces coelicolor | − | − | − | 2 |
| Corynebacterium glutamicum | − | − | − | 7 |
| Pseudomonas putida KT2440 | + | + | − | 18 |
| Pseudomonas putida DOT-T1E | + | + | − | 25 |
| Escherichia coli | + | + | − | 3 |
| Agrobacterium tumefaciens | + | + | − | 42 |
| Mesorhizobium loti | + | + | − | 8 |
| Clostridium acetobutylicum | − | − | − | 19 |
| Deinococcus radiodurans | − | − | − | 40 |
a
Bacterial strains were obtained from culture collection centers (DSMZ [German Collection of Microorganisms and Cell Cultures] and MBIC [Marine Biotechnology Institute Culture collection]). DNA was extracted from these strains as described elsewhere (14).
b
+, Strain known to have HAE1 gene(s); −, strain known to have no HAE1 gene.
c
+, Expected-size fragment was amplified; −, expected-size fragment was not amplified.