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. 2017 May 21;23(19):3440–3448. doi: 10.3748/wjg.v23.i19.3440

Figure 1.

Figure 1

Western blot analysis of AXL protein levels in cancer cell lines. A: Colon cancer cell lines HCT116, HCT116.p53, RKO, RKO.p53-/- and SW480. A band of 140 kDa was observed in AXL positive samples. Actin was used as a loading control; B: This graph shows the quantification of band intensity in comparison to Actin using the “ImageJ” program; value analysis was done using MS Excel. Red font indicates p53 mutation; C: Breast cancer cell line MCF7, MCF7-TP53 mutant clone 1001, ZR-75-1, CAL-51, MDA-MB-231, BT-549, MDA-MB-361, and T47D. HeLa was used as positive control for EMT. Actin was used as a loading control; D: This graph shows the quantification of band intensity in comparison to Actin using the “ImageJ” program and value analysis was done using MS Excel. Red font indicates p53 mutation.