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. 2017 May 23;7:2275. doi: 10.1038/s41598-017-02573-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of AMC on cell cycle arrest of HepG2 cells. Representative flow cytomeric analysis for the DNA content in HepG2 cells treated with AMC at different concentrations of 50 μg/mL, 100 μg/mL, and 200 μg/mL. Cell cycle distribution and cell number percentage in each phase (G0/G1, S, and G2/M) were detected and calculated (A). The protein expression of CDK1, CDK2, and Cyclin D1 were analyzed by western blot (B). Values were presented as mean ± SD for at least three independent experiments performed in triplicate. *p < 0.05 and **p < 0.01 versus the control group.