Bacteroides ovatus V975 |
Wild type |
B. ovatus strain V975 was obtained from T.R. Whitehead (USDA ARS NCAUR, Peoria, IL) |
Bacteroides ovatus GH439 |
ΔthyA, cblA::tetQ
|
This work |
Bacteroides ovatus GH440 |
ΔthyA, cblA::tetQ, ΔoxyR, BACOV975_00918::tgfβ_ex1
|
This work |
E. coli strain HB101 pRK201329
|
F–, thi-1, hsdS20 (rB
−, mB
−), supE44, recA13, ara14, leuB6, proA2, lacY1, galK2, rpsL20 (strr), xyl-5, mtl-1. pRK2013 (KmR, oriColE1, RK2-Mob+, RK2-Tra+) |
Clontech |
E. coli GC10 |
F−, mcrA, ∆(mrr-hsdRMS-mcrBC), Φ80d, lacZ∆M15, ∆lacX74, endA1, recA1, ∆(ara, leu) 7697araD139 galU, galK, nupG, rpsL, λ−, T1R. |
GeneChoice, Inc. |