Table 1.
Variations in sequences between published F. succinogenes S85 and re-sequencing of F. succinogenes S85 in this study.
| Number | Coordinate on CP001792 | Locus Tag | Annotation | ORF direction | Position at ORF sequence | Sequence on CP001792 | Sequence determined in this study | Count | Coverage | Frequency | Average quality | Distance to initiation codon | residue change |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Coding region | |||||||||||||
| 1 | 1007759 | Fisuc_0822 | FG-GAP repeat protein | — | 3670 | C | T | 115 | 116 | 99.1 | 36.87 | Val1224Ile | |
| 2 | 1007760 | Fisuc_0822 | FG-GAP repeat protein | — | 3669 | C | G | 115 | 116 | 99.1 | 36.87 | Thr1223, synonymous | |
| 3 | 1249999 | Fisuc_1012 | AzlC family protein | — | 142 | T | C | 179 | 237 | 75.5 | 37.483 | Thr48Ala | |
| 4 | 2498380 | Fisuc_2022 | Hypothetical | + | 2847 | C | T | 102 | 282 | 36.2 | 38.126 | Asp949, synonymous | |
| 5 | 2498437 | Fisuc_2022 | Hypothetical | + | 2904 | T | G | 123 | 265 | 46.4 | 35.132 | Thr968, synonymous | |
| 6 | 3746851 | Fisuc_3045 | Tryptophan synthase subunit | + | 1029 | G | T | 155 | 207 | 74.9 | 36.766 | Ala343, synonymous | |
| Noncoding region | |||||||||||||
| 1 | 2842868 | Intergenic (Fisuc_2307-Fisuc_2308) | — | A | 200 | 217 | 92.2 | 37.715 | 45, 141 | ||||
| 2 | 3495925 | Intergenic (Fisuc_2832, Fisuc_2833) | — | T | 144 | 160 | 90.0 | 37.521 | N/A | ||||