Table 2.
Sequence variations between the acid tolerant strain and wild type of F. succinogenes S85.
| Number | Coordinate on CP001792 | Locus Tag | Gene name | Annotation | ORF direction | Position at ORF sequence | Sequence on CP001792 | Sequence determined in this study | Count | Coverage | Frequency | residue change | Gene homolog in E. coli |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 612124 | Fisuc_0527 | sufD | ABC-type transport system involved in Fe-S cluster assembly, permease component | + | 514 | C | T | 61 | 151 | 63.04 | Arg172Cys | JW1671 (b1681) |
| 2 | 1396377 | Fisuc_1133 | yidE | Predicted permease, may bind an unidentified ligand | − | 462 | G | − | 200 | 200 | 100 | Frameshift. Stop codon at the 18th codon after the shift. | JW3662 (b3685) |
| 3 | 2230314 | Fisuc_1804 | xylE | sugar transporter | − | 205 | C | T | 217 | 220 | 96.14 | Ala69Thr | JW3991 (b4031) |
| 4 | 2399135 | Fisuc_1945 | rlmM (ygdE) | DNA alkylation repair | + | 331 | A | G | 199 | 199 | 94.12 | Thr111Ala | JW2777 (b2806) |
| 5 | 2561445 | Fisuc_2074 | mscL | Large conductance mechanosensitive channel protein. Critical roles in transducing physical stresses at cell membrane into an electrochemical response | − | 310 | T | C | 156 | 182 | 93 | Met104Val | JW3252 (b3291) |
| 6 | 2561669 | Fisuc_2074 | mscL | See above | − | 86 | C | A | 206 | 206 | 100 | Gly29Val | JW3252 (b3291) |
| 7 | 3649525 | Fisuc_2957 | dosC | Signal transducer; phosphorylation receiver or oxygen sensing | + | 803 | G | T | 230 | 232 | 100 | Gly268Val | JW5241 (b1490) |