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. 2017 May 23;7:2255. doi: 10.1038/s41598-017-01828-8

Figure 6.

Figure 6

Increased expression of pro-inflammatory cytokines in aortas of Apoe −/− Il27ra −/− mice. (A,C) Relative gene expression of IFNγ, TNF-α and IL-17A in aortas of Apoe −/− Il27ra −/− (n = 5) mice fed with WD for 7 weeks (A) or 18 weeks (C) were normalized to L-32 gene expression and fold induction was calculated based on the gene expression in aortas of control Apoe −/− Il27ra +/− (n = 5). (B,D) Production of pro-inflammatory cytokines IFNγ, TNF-α and IL-17A in aortas of Apoe −/− Il27ra +/− or Apoe −/− Il27ra −/− mice at different stages of atherosclerosis was measured by multiplex cytokines array in supernatants of aortic cell suspension obtained from Apoe −/− Il27ra +/− (n = 5) or Apoe −/− Il27ra −/− (n = 5) mice fed with WD for 7 (B) or 18 weeks (D), stimulated with anti-CD3/anti-CD28 for 48 hours. Data are mean ± SEM from 3 independent experiments.