Skip to main content
. 2005 Jan;71(1):51–57. doi: 10.1128/AEM.71.1.51-57.2005

TABLE 3.

Plasmid transfer frequencies for matings between P. putida CAP8(pTOL::Pa1/04/03 gfpmut3b) and P. aeruginosa PAO1162N or PAO2002N that was previously exposed to SDSa

Recipient Concn (mM) of SDS Di/Ri Di + Ri (CFU [103]/mm2) Df + Rf (CFU [103]/mm2) T/(DiRi) (10−10) (events/mm2)−1 C/(DiRi) (10−10) (events/mm2)−1
PAO2002N 0 0.08 400 ± 56 5,800 ± 1,400 ND 0.078 ± 0.0062
1 0.12 5,900 ± 3,326 ND 0.154 ± 0.029
2 0.09 5,600 ± 1,170 ND 0.140 ± 0.094
4 0.06 5,900 ± 3,200 ND 0.089 ± 0.063
7 0.06 6,500 ± 1,990 ND 0.51 ± 0.043
10 0.05 6,100 ± 3,400 0.104 ± 0.036 0.116 ± 0.029
PAO1162N 0 13 146 ± 2.1 400 ± 150 2,700 ± 640
1 43 580 ± 113 17,600 ± 12,200
4 378 2,300 ± 149 92,000 ± 44,000
7 378 2,800 ± 490 240,000 ± 65,000 0.37 ± 0.051
10 >1,970 2,800 ± 690 600,000 ± 480,000 0.78 ± 0.163
0 20 41.9 ± 4.7 347 ± 21 88 ± 42 1.36 ± 0.73
1 3.5 66.5 ± 6.2 15.8 ± 4.8 0.154 ± 0.044
4 31 199 ± 9.8 148 ± 69 0.96 ± 0.034
7 9.3 326 ± 63 86 ± 16.2 0.43 ± 0.157
10 3.3 383 ± 38 21.0 ± 5.1 0.154 ± 0.018
a

C, T, Df, and Rf were enumerated after 48-h mating incubations. The initial total density was 1.50 × 107 CFU/mm2; all values are means±standard deviations; values shown in bold are statistically different from mating results with unexposed recipients. ND, not detected.