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. 2017 May 23;7:2278. doi: 10.1038/s41598-017-02642-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of HpSlyD (stable cell lines) leads to increased expression of CDX2 and VIL1 in AGS cells. (A,B) Fold increase in CDX2 (A) and VIL1 (B) mRNA expression in AGS cells and AGS cells expressing either HpSlyD-GFP or GFP alone. The values obtained with the AGS cells are referred to as 1. CDX2 and VIL1 mRNA levels are normalized to GAPDH mRNA. (C,D,E) Western blots for CDX2 (C,D) and VIL1 (C,E) expression in AGS cells and AGS cells expressing either HpSlyD-GFP or GFP alone. AGS cells were used as a control and GAPDH was used as a loading control. The results shown in (A,B,D,E) are means ± SD, each experiment performed in triplicate. *P < 0.05, **P < 0.01 as compared with AGS cells.