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. 2017 Mar 14;230(6):775–786. doi: 10.1111/joa.12603

Figure 4.

Figure 4

Effect of estrous cycle on the kisspeptin immunoreactivity (kiss‐ir) in adult mice. (A) The representative subdivision of the paraventricular nucleus (PVN) in four quadrants (DM, dorso‐medial; DL, dorso‐lateral; VM, ventro‐medial; VL, ventro‐lateral). (B, C) The comparison of kiss‐ir fibers within the PVN of female CD1 mice in estrus (B) and diestrus (C) phases, respectively. (D) Histograms representing the fractional area covered by kiss‐ir structures (mean ± SEM) in the PVN (DM, DL, VM, VL), anterior ventral periventricular nucleus (AVPV) and arcuate hypothalamic nucleus (ARC) of female adult mice in estrus (dark gray) and diestrus (light gray) phases. *P < 0.05, ***P < 0.001 different from diestrus (P < 0.05, Bonferroni test). Scale bar: 50 μm.