Table 1.
Primary and secondary antibodies used in the double‐label immunofluorescence assays
| Primary Abs | |||||
| Kisspeptin | AC053 | A. Caraty | Sheep, pc | 1 : 2000 | Poling et al. (2013) |
| NkB | T4450 | Peninsula | Rabbit, pc | 1 : 2000 | Taziaux et al. (2012) |
| AVP | 64717 | ICN | Rabbit, pc | 1 : 8000 | Ferris et al. (1997) |
| nNOS | 24287 | DiaSorin | Rabbit, pc | 1 : 3000 | Gillespie et al. (2005) |
| OT | AB911 | Millipore | Rabbit, pc | 1 : 8000 | Bean et al. (2014) |
| TH | 22941 | Incstar | Mouse, mc | 1 : 8000 | Daadi & Weiss (1999) |
| Secondary Abs | |||||
| Anti‐sheep Alexa Fluor® 555 | A21436 | Invitrogen | Donkey, pc | 1 : 500 | |
| Anti‐rabbit Alexa Fluor® 488 | A21206 | Invitrogen | Donkey, pc | 1 : 500 | |
| Anti‐mouse Alexa Fluor® 488 | A21202 | Invitrogen | Donkey, pc | 1 : 500 | |
AVP, arginine vasopressin; mc, monoclonal antibody; NkB, neurokinin B; nNOS, neural nitric oxide synthase; OT, oxytocin; pc, polyclonal antibody; TH, tyrosine hydroxylase.